EGFR (Phospho-Tyr1172)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie designed for the qualitative measurement of phosphorylated Epidermal Growth Factor Receptor (EGFR) at tyrosine residue 1172 in cultured adherent cells. The assay is reactive with Human, Mouse, and Rat samples and uses a colorimetric detection method measured at 450 nm. Described as lysate-free and high-throughput, it is intended for measuring relative levels of phosphorylated EGFR in cultured cells and for screening the effects of treatments, inhibitors such as siRNA or chemicals, and activators on EGFR phosphorylation.

Detection uses an indirect ELISA format in which EGFR (Phospho-Tyr1172)-specific primary antibodies are paired with HRP-conjugated secondary antibodies; the conjugated HRP catalyses a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance. Following the colorimetric measurement, the Crystal Violet whole-cell staining method may be used to determine cell density, with absorbance values normalised to cell amounts to adjust for plating differences. The stated dynamic range is > 5000 Cells. The kit is supplied in a 96-well microplate format. Associated database entries include Gene ID: 1956, UniProt ID: P00533, OMIM: 131550/211980, and Unigene: Hs.488293.