EGFR (Phospho-Tyr1110)Colorimetric Cell-Based ELISA Kit

The Assay Genie cell-based ELISA kit offers a colorimetric (450 nm) method for measuring phosphorylation of EGFR at tyrosine 1110 in adherent cells. It is a lysate-free, high-throughput assay run in an indirect ELISA format: phospho-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition.

Validated for Human, Mouse, and Rat samples, the assay can be used to quantify relative levels of phosphorylated EGFR in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), and activators on receptor activation. The reported dynamic range is > 5000 Cells. Because the readout is qualitative, two normalization strategies are provided: an anti-GAPDH monoclonal antibody serves as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density so absorbance values can be normalized to cell amount.

EGFR is a single-pass transmembrane receptor tyrosine kinase that regulates cell growth, proliferation, differentiation, and survival, and phosphorylation at residues such as Tyr1110 drives downstream signaling cascades linked to cancer-related processes. The kit is positioned for research into EGFR signaling, tumorigenesis mechanisms, and drug resistance in oncology and cell biology.