EGFR (Phospho-Thr693)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the accurate detection of phosphorylated EGFR (Epidermal Growth Factor Receptor) at threonine 693 in cell lysates or tissue homogenates. The assay is lysate-free and high throughput, allowing measurement of relative amounts of phosphorylated EGFR in cultured cells as well as screening for the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on EGFR phosphorylation.

Qualitative determination of EGFR (Phospho-Thr693) is achieved via an indirect ELISA format, in which phospho-specific primary antibodies capture the target while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction measured at 450 nm. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method can be used to determine cell density so absorbance values can be normalized to cell amounts. The kit is reactive with Human, Mouse and Rat samples, with adherent cells as the indicated sample type, and has a stated dynamic range of more than 5000 cells. Gene ID: 1956, UniProt ID: P00533.