EFNB3 Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie detects the EFNB3 (Ephrin-B3) protein expression profile in cultured cells, with a colorimetric readout measured at 450 nm. The assay uses an indirect ELISA format in which EFNB3 is captured by target-specific primary antibodies while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP enzyme to catalyze a colorimetric reaction upon substrate addition. The kit is described as lysate-free, high throughput, and sensitive, with a stated dynamic range of > 5000 cells, and is intended for use with adherent cell samples. Reactivity is reported for human, mouse, and rat.

A monoclonal anti-GAPDH antibody is included to serve as an internal positive control for normalizing target absorbance values. A Crystal Violet whole-cell staining method is also provided to determine cell density, allowing absorbance values to be normalized to cell amounts and correcting for plating differences. The kit can be used to measure the relative amounts of EFNB3 in cultured cells and to screen the effects of treatments, inhibitors such as siRNA or chemicals, or activators on EFNB3 expression. Because the determination is qualitative in nature, these multiple normalization methods are required.

EFNB3 is a cell-surface transmembrane ligand for Eph receptors and is implicated in cell signaling, neuronal development, axon guidance, and forebrain function. Its dysregulation has been linked to neurological disorders including Alzheimer's disease and schizophrenia, making the kit a relevant research tool for studies of cell signaling pathways and neuroscience. Storage is at 4°C with a stated stability of 6 months.