EFNB1 (Phospho-Tyr317)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the qualitative detection of EFNB1 (Ephrin-B1) phosphorylation at the Tyr317 residue directly in cultured cells. Using an indirect ELISA format, phosphorylated EFNB1 (Tyr317) is captured by phospho-specific primary antibodies while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling a colorimetric readout at 450 nm. The kit is positioned for signalling-pathway studies in developmental biology, cancer biology, and neurobiology, where Tyr317 phosphorylation is implicated in EFNB1-mediated cell adhesion, migration, and differentiation.

Reactivity has been validated for human, mouse, and rat samples, and the assay is suited to adherent cell cultures with a stated dynamic range of > 5000 Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step is provided so that absorbance readings can be normalised to cell density and plating differences adjusted. The workflow can be used both to measure relative levels of phosphorylated EFNB1 in cultured cells and to screen the effects of various treatments, inhibitors (e.g., siRNA or chemicals), or activators on EFNB1 phosphorylation.