EFNB1 Colorimetric Cell-Based ELISA Kit

Designed for the accurate and reliable detection of EFNB1 (Ephrin B1) levels in cell samples, this Cell-Based ELISA Kit from Assay Genie is offered for Human, Mouse, and Rat reactivity. The kit uses a colorimetric detection method read at 450 nm, with a stated dynamic range of > 5000 Cells, and is configured for adherent cell samples in a lysate-free, high throughput format.

The assay follows an indirect ELISA approach: EFNB1 is captured by EFNB1-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP to catalyse a colorimetric reaction on substrate addition. It can be used to measure relative amounts of EFNB1 in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on EFNB1. Because the readout is qualitative, two normalisation strategies are built in: a monoclonal anti-GAPDH antibody serves as an internal positive control for normalising target absorbance, and Crystal Violet whole-cell staining can be used to determine cell density and adjust for plating differences.

Storage is specified at 4 °C with a stated stability of 6 Months. The product is associated with the Developmental Biology research area and targets EFNB1, which encodes a type I membrane protein of the ephrin family that acts as a ligand of Eph-related receptor tyrosine kinases and has been linked to cell adhesion, cell-to-cell communication, and nervous system development or maintenance.