EFNA4 Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed for the accurate detection of EFNA4 (ephrin-A4) levels in cell lysates and cell culture supernatants, with reactivity for both human and mouse samples. The assay uses a colorimetric detection method read at 450 nm and has a stated dynamic range of > 5000 Cells, offering a convenient, lysate-free, high-throughput format for measuring relative EFNA4 protein expression in cultured cells.

Detection follows an indirect ELISA format: EFNA4 is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Substrate addition produces a colorimetric readout. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be normalized to cell numbers. The kit is supplied in a 96-Well Microplate format, is stored at 4°C for up to 6 months, and is suited to screening the effects of treatments, inhibitors (such as siRNA or chemicals), and activators on EFNA4.

EFNA4 (ephrin-A4) is described as a cell-surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases important in migration, repulsion and adhesion during neuronal, vascular and epithelial development. The protein is reported to play roles in cell adhesion, migration and differentiation, and dysregulation of EFNA4 has been linked to cancer, inflammatory disorders and neurodegenerative diseases, making it a biomarker of interest for studying disease mechanisms and potential therapeutic interventions.