Durvalumab (Imfinzi®)Free drug ELISA Kit

This colorimetric ELISA kit from Assay Genie is designed to measure free durvalumab in human serum and plasma. It uses a solid-phase enzyme-linked immunosorbent assay based on the sandwich principle, with standards and samples incubated in a microtiter plate coated with the reactant for durvalumab. After the wells are washed, an HRP-conjugated probe is added; following further incubation and washing, bound enzymatic activity is detected with TMB chromogen substrate, and the reaction is terminated with an acidic stop solution. The developed color is proportional to the amount of durvalumab in the sample or standard, and results can be determined directly using the standard curve.

The listed application is “Free Drug,” and the research area is “Checkpoint Inhibitors.” The required sample volume is “10 µL,” and the listed total time is “70 Minutes.” The stated detection limit is “30 ng/mL,” the spike recovery is “85-115%,” and the shelf life is “1” year.