Dolphin IL-1 RA DIY ELISA Kit

The Dolphin IL-1 RA DIY ELISA Kit from Assay Genie is configured for quantifying interleukin-1 receptor antagonist, a competitive antagonist of IL-1α and IL-1β receptors that serves as a central regulator of inflammatory responses in marine mammals. The modular DIY format provides separate vials of capture antibody, biotinylated detection antibody, recombinant dolphin IL-1 RA standard, and streptavidin-HRP, allowing laboratories to build custom plate layouts across up to 10 microplates.

Standards are calibrated against a recombinant dolphin IL-1 RA reference, with a working assay range of 15.6 to 1000 pg/mL and a minimum detectable concentration of approximately 5 pg/mL. The recommended sample volume is 100 uL per well. Capture antibody, detection antibody, and standard should be stored at -20 °C and brought to room temperature immediately before use, while the chromogenic substrate is kept refrigerated and protected from light. Intended for research use only and in vitro applications, the kit supports studies of immune regulation, stress response, and infectious disease in cetacean populations.