Dolphin CCL2 DIY ELISA Kit

The Dolphin CCL2 DIY ELISA Kit from Assay Genie provides a research-use-only immunoassay for measuring monocyte chemoattractant protein-1 (also called CCL2 or MCP-1), a pro-inflammatory chemokine that directs monocyte and T-cell recruitment during immune responses. In cetacean biology, CCL2 levels serve as a biomarker in studies of marine mammal health, inflammatory disease, and responses to environmental or pathogenic stress. This DIY format supplies the core reagents, pre-titrated antibodies, and standards so the user assembles the assay on their own microplate, allowing flexible batch sizes up to ten plates per kit. Reconstituted standards and diluted samples should be handled on ice and protected from light, while the kit components are stored refrigerated at 4 degrees C until first use. Sample volumes around 100 µL per well are recommended, with absorbance measurements on a standard plate reader at the wavelength specified in the protocol.