Dog High mobility group protein B1 (HMGB1) ELISA Kit

A Sandwich ELISA Kit from Assay Genie designed for the quantitative measurement of High mobility group protein B1 (HMGB1) in canine samples. The kit uses a colorimetric detection method and is reactive with dog samples, supporting research applications in inflammation, immune response, and tissue repair processes. HMGB1 is a key biomarker associated with conditions including cancer, sepsis, and autoimmune disorders, making this kit valuable for veterinary medicine research.

The kit accepts serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, with specificity for natural and recombinant dog HMGB1. Detection range is 0.156-10ng/mL with a sensitivity of 0.056ng/mL. The target protein (UniProt Q6YKA4, NCBI Gene ID 403170) is a DNA-binding chromatin-associated protein involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair, and acts as a danger-associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. It also functions as a cofactor of the RAG complex during V(D)J recombination and has heparin-binding and proangiogenic activity. The protein localises to the nucleus and chromosome, and falls within the epigenetics research area. For research use only.