Dog Apolipoprotein A-II (APOA2) ELISA Kit

Apolipoprotein A-II (APOA2) is the second major apolipoprotein constituent of high-density lipoprotein (HDL) particles and contributes to reverse cholesterol transport, HDL remodeling, and triglyceride metabolism. In canine models, circulating APOA2 concentrations are investigated in research on dyslipidemia, obesity-associated metabolic changes, and cardiovascular risk, so this Assay Genie Sandwich ELISA is a practical tool for veterinary and comparative cardiovascular investigation.

The kit uses a pre-coated 96-well microplate and a colorimetric detection method read at 450 nm with a standard microplate reader. Capture and detection antibody pairs recognize canine APOA2 in serum and plasma samples, with incubation steps typically performed at 37 °C before stop solution is added and absorbance is measured. Reconstituted standards and biotinylated antibodies should be stored refrigerated at 2-8 °C to preserve activity; unused strips can be kept in the resealed foil pouch with desiccant for short-term storage. The 96 assays format provides wells for duplicate standards, controls, and multiple samples, supporting research use only applications in lipid and metabolic studies.

Each Assay Genie APOA2 kit ships with a pre-titrated standard that lets researchers generate a 7-point standard curve, typically spanning roughly 0.156–10 ng/mL, against which unknown canine serum or plasma samples are interpolated. The capture antibody is coated on a removable 96-well microplate (12 strips of 8 wells), so partially used plates can be resealed in the foil pouch with desiccant and held at 2-8 °C for short-term reuse. Sample volume per well is generally 50 µL after dilution, and the full protocol — including a 37 °C incubation step and biotinylated detector incubation — can be completed in roughly 3.5 hours, which is convenient for laboratories running multiple plates per week.

Reported intra-assay and inter-assay coefficients of variation for this sandwich ELISA format typically fall below 10%, supporting reproducible quantification of canine APOA2 across runs. Researchers studying obesity-related metabolic dysfunction, insulin resistance, and atherosclerosis in dog models use the kit to track circulating APOA2 alongside lipids such as HDL-C, LDL-C, and triglycerides. Reconstituted working standards and streptavidin-HRP conjugates should be kept refrigerated at 2-8 °C, and longer-term storage of unused reagents at -20 °C is recommended to preserve antibody activity over repeated freeze-thaw cycles. As with all Assay Genie immunoassays, components are intended for research use only and are not validated for diagnostic or clinical decision-making in companion animals.