DNA Polymerase beta Colorimetric Cell-Based ELISA Kit

A cell-based colorimetric ELISA from Assay Genie enables relative quantification of DNA Polymerase beta protein in cultured adherent cells. The kit is lysate-free and uses an indirect ELISA format, in which target-specific primary antibodies are bound by HRP-conjugated secondary antibodies that catalyze a colorimetric reaction read at 450 nm. It is designed for high-throughput screening of treatments, inhibitors (such as siRNA or chemicals), and activators that affect DNA Polymerase beta expression.

Validated reactivity covers human, mouse, and rat samples, with a dynamic range of > 5000 cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance, and a Crystal Violet whole-cell staining step can be used to adjust absorbance values to cell density. The assay is supplied in a 96-well microplate format, with storage at 4°C and a shelf stability of 6 months.

DNA polymerase beta (gene symbol POLB; UniProt P06746) is a repair polymerase central to base-excision repair, with reported 5'-deoxyribose-5-phosphate (dRP) lyase activity and a stepwise, distributive gap-filling mode of DNA synthesis. The protein has a reported molecular weight of ~ 38kDa. The kit is grouped within the Epigenetics and Nuclear Signaling research area and supports studies in molecular biology, genetics, and disease research where this enzyme's role in maintaining genomic integrity is of interest.