DLX5 Transcription Factor Activity Assay

The DLX5 Transcription Factor Activity Assay from Assay Genie is a DNA-binding immunoassay that quantifies the DNA-binding activity of the DLX5 homeobox transcription factor in nuclear and cell lysates. The microplate is pre-coated with double-stranded oligonucleotides containing a consensus DLX5 response element, and activated DLX5 captured from the sample is detected with a transcription factor-specific primary antibody. Researchers apply this kit to monitor DLX5 activation during osteoblast differentiation, chondrogenesis, and craniofacial development studies, as well as downstream signaling related to RUNX2 and BMP pathways. Each kit supplies reagents for 96 individual reactions in a single well plate format. Store the unopened kit at -20 degrees C, protect from light, and avoid repeated freeze-thaw cycles; equilibrate components to room temperature before running the assay. Intended for research use only and compatible with lysates prepared from human, mouse, and rat cells.