DLX4 Colorimetric Cell-Based ELISA

From Assay Genie, this Colorimetric Cell-Based ELISA kit is designed for the qualitative determination of DLX4 protein expression in cultured cells, working with cell lysates and tissue homogenates. The indirect ELISA format uses DLX4-specific primary antibodies captured on the plate, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. Detection is read at 450 nm, and the assay is reactive with human samples.

The kit employs a lysate-free, high-throughput protocol with a dynamic range of > 5000 Cells. For normalisation, a monoclonal antibody specific for human GAPDH is included as an internal positive control in normalising target absorbance values, while Crystal Violet whole-cell staining may be used to determine cell density and adjust for plating differences. The assay is supplied in a 96-Well Microplate format and is intended for measuring the relative amounts of DLX4 in cultured cells, as well as screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on DLX4.

DLX4 is a homeobox transcription factor encoded by the DLX4 gene (Gene ID: 1748, UniProt: Q92988), with reported roles in regulating gene expression during embryonic development. Dysregulation of DLX4 has been implicated in cancer, developmental disorders, and neurological conditions, making it a valuable biomarker for studying these diseases. The kit is stored at 4°C with a stated stability of 6 Months.