DLX3 Transcription Factor Activity Assay

The DLX3 (Distal-Less Homeobox 3) activity assay from Assay Genie uses a microplate format pre-coated with a double-stranded oligonucleotide containing the DLX3 consensus DNA binding motif. Activated transcription factor present in nuclear or whole-cell lysates recognizes the immobilized probe, and bound DLX3 is detected with a primary antibody followed by incubation with a substrate. Each kit supports 96 reactions and includes reagents verified for use with human, mouse, and rat samples.

Researchers apply this kit to study DLX3 function in amelogenesis, craniofacial bone formation, and epidermal differentiation, using it as a quantitative alternative to EMSA gels for measuring transcription factor activation in cell-based experiments. The kit components should be stored refrigerated at 4°C, with antibody and probe aliquots kept frozen at -20°C to maintain binding activity across multiple freeze-thaw cycles.