DLX3 Colorimetric Cell-Based ELISA

A cell-based ELISA kit from Assay Genie designed to detect the DLX3 transcription factor in cultured cells. The assay is a convenient, lysate-free, high-throughput and sensitive method for measuring relative DLX3 expression, and is suitable for screening the effects of various treatments, inhibitors or activators on DLX3 levels. Qualitative determination is achieved by an indirect ELISA format in which DLX3 is captured by DLX3-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the HRP enzyme to catalyse a colorimetric reaction upon substrate addition.

The kit is reactive with Human and Mouse samples and is intended for adherent cells. Detection is colorimetric at 450 nm, with a stated dynamic range of > 5000 Cells, supplied in a 96-Well Microplate format and stored at 4°C for 6 Months. Normalisation is supported by an included anti-human GAPDH monoclonal antibody acting as an internal positive control, and by a Crystal Violet whole-cell staining method to determine cell density. DLX3 (Gene ID: 1747, UniProt ID: O60479) is a DNA-binding transcription factor of the distal-less homeobox family with a molecular weight of 31,738 Da; the human ortholog maps to chromosome 17q21.33, and mutations are associated with Trichodentoosseous Syndrome and Amelogenesis Imperfecta Type IV.