DLGP1 Colorimetric Cell-Based ELISA

A Cell-Based ELISA Kit from Assay Genie offers a convenient, lysate-free, high-throughput approach for detecting DLGP1 protein expression profiles in cultured cells. The assay uses an indirect ELISA format in which DLGP1 is captured by target-specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction read at 450 nm. The kit can be used to measure relative amounts of DLGP1 in cultured cells and to screen for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on DLGP1 expression.

DLGP1, also referred to as SAP97, is a scaffolding protein involved in synapse formation and function in the brain, contributing to the regulation of neural development, synaptic plasticity, and cognitive function. Dysregulation of DLGP1 has been implicated in neurological disorders including schizophrenia, autism, and Alzheimer's disease, making it a useful biomarker for related research. Two normalisation approaches are built into the workflow: a monoclonal antibody specific for human GAPDH serves as an internal positive control, and a Crystal Violet whole-cell staining method may be used to determine cell density so absorbance values can be adjusted for plating differences.

The kit is reactive with Human, Mouse, and Rat samples and is designed for use with adherent cells. It has a dynamic range of > 5000 Cells, is supplied in a 96-well microplate format, uses colorimetric detection at 450 nm, and is stored at 4°C with a stated stability of 6 months.