DJ-1 Colorimetric Cell-Based ELISA Kit

Assay Genie offers a cell-based ELISA kit designed for the qualitative detection of DJ-1 protein expression directly in cultured cells. The assay uses an indirect ELISA format, in which DJ-1-specific primary antibodies capture the target and HRP-conjugated secondary antibodies catalyse a colour-forming reaction upon substrate addition, with colorimetric readout at 450 nm.

Reactive with human and mouse samples and intended for use with adherent cells, the system supports a dynamic range of more than 5000 cells. A monoclonal GAPDH antibody is included as an internal positive control to normalise target absorbance, and a Crystal Violet whole-cell staining step can be used to correct for cell density differences across wells. The kit is supplied in a 96-well microplate format and is stored at 4 °C with a stated stability of 6 months.

DJ-1 (gene symbol PARK7) is a protein implicated in oxidative stress response, mitochondrial function and neuroprotection, and is studied as a biomarker in Parkinson's disease, cancer and other neurodegenerative disorders. The platform is suited to measuring relative DJ-1 levels in cultured cells and to screening the effects of treatments, inhibitors or activators on DJ-1 expression.