Dihydrotestosterone (DHT) ELISA Kit

From Assay Genie, this Competitive ELISA Kit is engineered for the detection and quantitative measurement of dihydrotestosterone (DHT), with general reactivity that supports use across a broad range of sample types. It is intended for research use only and is validated to deliver consistent and reproducible results with high sensitivity and specificity. DHT is a potent androgen hormone that plays a critical role in male sexual development and reproductive function, and is implicated in conditions such as prostate cancer and male pattern baldness, making it a valuable biomarker for the study of these physiological and pathological processes.

Designed to work with serum and plasma samples, the kit offers supported protocols for additional matrices including cell culture supernatants, urine, cerebrospinal fluid, cell lysates, tissue homogenates, tissue lysates, and breast milk. It operates across a measurement range of 0.156-10 ng/mL in a competitive ELISA format. The kit includes a dismountable ELISA microplate, lyophilized standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate solution, stop solution, and plate sealers; most components are stored at -20°C, with wash buffer, substrate, and stop solution stored at 4°C.

The assay is read on a microplate reader set to 450 nm, following a stepwise protocol of standard/sample addition, detection reagent incubation, washing, substrate development, and stop reaction, as described in the kit insert.