DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit (UNES00012)

A colorimetric competitive ELISA from Assay Genie is designed for the quantitative measurement of 1,25-Dihydroxyvitamin D3 (DHVD3), also known as calcitriol, in serum, plasma and other biological fluids. The assay uses a Competitive-ELISA format with a total run time of 2 h 30 min and a sample volume of 50 μL. It is intended for research applications in metabolism and developmental biology.

Performance characteristics include a sensitivity of 4.69 pg/mL, a detection range of 7.81–500 pg/mL and a reported recovery of 80%–120%. Reactivity is described as universal, with no significant cross-reactivity or interference observed between universal DHVD3 and analogues in samples. The kit components are stored at 2–8°C.