Deoxypyridinoline (DPD) ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the quantification of deoxypyridinoline (DPD) in biological samples. Deoxypyridinoline is described as a key biomarker for bone health and turnover, with elevated levels often indicating increased bone resorption, supporting research into bone metabolism, osteoporosis, and other bone-related disorders. The kit is for research use only.

Validated for serum and plasma samples with general reactivity, the assay uses a competitive detection format and reports a measurement range of 1.56–100 ng/mL. Supplied components include a dismountable ELISA microplate, lyophilized standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate, stop solution, and plate sealers, each with its own storage temperature.

The workflow involves adding sample and detection reagent working solutions to the plate, incubation steps at 37 °C, repeated wash cycles, addition of substrate solution, termination with stop solution, and measurement of optical density at 450 nm using a microplate reader.