Dematin (Phospho-Ser403)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the precise detection of dematin levels in cell lysates and tissue homogenates, targeting the phosphorylated form at Ser403. The kit uses a colorimetric detection method read at 450 nm and is reactive with Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells.

Working with adherent cells, the assay is lysate-free and high-throughput, employing an indirect ELISA format in which phosphorylated Dematin is captured by phospho-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. It enables measurement of relative phosphorylated Dematin levels in cultured cells and screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Dematin phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control in normalizing the target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density.

The kit targets the Dematin protein (gene symbol EPB49, Gene ID 2039, UniProt ID Q08495, OMIM 125305, chromosomal location 8p21.1), an actin-binding and bundling protein with a reported molecular weight of 41,558 Da, and is supplied by Assay Genie as a high-quality Cell-Based ELISA Kit.