Dehydroepiandrosterone (DHEA) ELISA Kit

Assay Genie's Dehydroepiandrosterone (DHEA) ELISA Kit is a competitive enzyme-linked immunosorbent assay designed for the quantitative detection of DHEA in serum, plasma, and cell culture supernatant samples. The assay uses a colorimetric detection method, with absorbance measured at 450 nm on a standard microplate reader, and is rated for general reactivity.

DHEA is a key precursor hormone involved in numerous physiological processes, including hormone production and regulation, immune function, and metabolism. Altered DHEA levels have been linked to conditions such as aging, adrenal insufficiency, and mood disorders, making it a valuable biomarker for research applications. The kit reports a measuring range of 0.312-20 ng/mL and is intended for research use only.

The supplied protocol involves pipetting standards, blanks, and samples into the coated microplate, sequential addition of detection reagents with incubation at 37°C, washing steps, substrate development, and stop solution, with final optical density values used to calculate DHEA concentrations.