DDR1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit provides a colorimetric method for measuring DDR1 protein levels in cultured cells. The assay is described as a convenient, lysate-free, high throughput and sensitive tool that detects the DDR1 expression profile in cells without requiring lysate preparation. It works in an indirect ELISA format, where DDR1 is captured by specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. DDR1 (discoidin domain receptor 1) is a receptor tyrosine kinase implicated in cancer progression, fibrosis and inflammatory disorders, and the kit supports research into its signaling pathways.

The kit is suitable for adherent cell samples and is reactive with human, mouse and rat species. It has a dynamic range of > 5000 cells and uses a colorimetric readout at 450 nm. Storage is at 4°C with a stated stability of 6 months. To support data normalization, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and a Crystal Violet whole-cell staining method can be used to determine cell density after staining.