DARPP-32 (Phospho-Thr75)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit enables detection of DARPP-32 phosphorylated on threonine 75 in cultured cells using an indirect ELISA format. HRP-conjugated secondary antibodies catalyze the colorimetric reaction, with absorbance read at 450 nm. The kit is reactive with human, mouse, and rat samples and is validated for adherent cells. It provides a lysate-free, high-throughput workflow and operates with a dynamic range of > 5000 Cells.

A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values. A Crystal Violet whole-cell staining method is also provided to determine cell density, allowing absorbance values to be normalized to cell amounts and plating differences to be adjusted.

DARPP-32 (Dopamine- and cAMP-regulated neuronal phosphoprotein) is a signaling molecule involved in neurotransmission and neuronal function. Phosphorylation at threonine 75 regulates its activity and is implicated in neurological disorders including Parkinson's disease and schizophrenia. By measuring phosphorylation at this site, researchers can study the signaling mechanisms involved in these disorders and explore potential therapeutic targets.