Dab1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA kit is designed to detect Dab1 (Disabled-1) protein expression directly in cells, using a colorimetric readout at 450 nm. The assay is a lysate-free, indirect ELISA format in which Dab1-specific primary antibodies are captured and detected with HRP-conjugated secondary antibodies, enabling qualitative measurement of relative Dab1 levels in cultured cells.

Reactivity covers human, mouse, and rat samples, and the dynamic range is stated as "> 5000 Cells". The kit supports adherent cell workflows and can be used to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on Dab1. For data normalization, an anti-GAPDH monoclonal antibody is included as an internal positive control, and Crystal Violet whole-cell staining is provided for determining cell density after the colorimetric measurement.

DAB1 is described as an adaptor molecule involved in neuronal migration and brain development, with a molecular weight of 64 kDa (UniProt ID: O75553). The kit is positioned for developmental biology research, with relevance to neurological conditions such as autism, schizophrenia, and Alzheimer's disease. Recommended storage is 4 °C with a 6-month stability period.