Cytochrome P450 3A43 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA Kit is designed for the accurate detection of Cytochrome P450 3A43 levels in cell lysates and tissue extracts, offering high sensitivity and specificity for a range of research applications. Cytochrome P450 3A43 is an enzyme involved in drug metabolism and detoxification processes in the liver, making it a key target for drug development and personalized medicine research.

The kit provides a convenient, lysate-free, high-throughput and sensitive assay that detects Cytochrome P450 3A43 protein expression profiles in cultured cells. It can be used to measure relative amounts of Cytochrome P450 3A43 and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators. Qualitative determination is performed using an indirect ELISA format in which the target is captured by Cytochrome P450 3A43-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyse a colorimetric reaction upon substrate addition.

Detection is read at 450 nm in a 96-well microplate format with a stated dynamic range of > 5000 Cells, and the kit is intended for use with adherent cells. Reactivity is human, and storage is at 4°C for 6 months. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance, and a Crystal Violet staining method may be used after the colorimetric measurement to determine cell density, allowing absorbance values to be normalised to cell amounts.