Cytochrome P450 21A2 Colorimetric Cell-Based ELISA

Designed for the accurate detection of cytochrome P450 21A2 (CYP21A2) in cell culture samples, this Cell-Based ELISA Kit from Assay Genie offers a colorimetric, lysate-free workflow suited to high-throughput research. The assay uses an indirect ELISA format in which target-specific primary antibodies capture the protein and HRP-conjugated secondary antibodies catalyze a colorimetric reaction measured at 450 nm.

Intended for use with adherent cells, the kit has a dynamic range of > 5000 Cells and includes an anti-GAPDH monoclonal antibody to serve as an internal positive control for normalization, together with a Crystal Violet whole-cell staining method for determining cell density. It is suited to measuring relative CYP21A2 expression in cultured cells and to screening the effects of treatments, inhibitors, or activators on the target, and is described as offering high sensitivity and specificity for reliable results.

Reactivity is reported for human samples, and the kit is stored at 4°C with a stated stability of 6 Months. The associated gene record is Gene ID: 1589 (UniProt ID: P08686), within the Metabolism research area.