Cysteine (Cys) ELISA Kit

Assay Genie offers a competitive ELISA kit designed for the accurate quantification of cysteine (Cys) in biological samples. Cysteine is described in source material as a vital amino acid with antioxidant properties involved in protein synthesis, detoxification, and immune function, with abnormal levels linked to oxidative stress, cardiovascular diseases, and neurological disorders.

The assay uses a colorimetric detection method with a stated range of 0.312-20 ng/mL and reads absorbance at 450 nm. It is validated for serum and plasma, and the manufacturer's protocol also covers preparation of cell culture supernatants, cell lysates, tissue homogenates, tissue lysates, urine, cerebrospinal fluid, and breast milk. Reactivity is listed as Universal, and the kit is intended for research use only.

Supplied components include a dismountable ELISA microplate, lyophilized standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate solution, stop solution, and plate sealers, with storage temperatures for each item specified in the kit insert. Additional materials required but not provided include a 450 nm microplate reader, multichannel pipette, incubator, and deionized or distilled water.