Cyclin H (Phospho-Thr315)Colorimetric Cell-Based ELISA Kit

From Assay Genie, the Cyclin H (Phospho-Thr315) Colorimetric Cell-Based ELISA Kit is a cell-based assay intended for adherent cell samples, with reactivity to Human, Mouse, and Rat. Designed for the reliable detection of phosphorylated cyclin H at threonine 315 in cell lysates, it offers high sensitivity and specificity for accurate and reproducible results.

The kit uses a lysate-free, indirect ELISA format in which Cyclin H (Phospho-Thr315) is captured by specific primary antibodies, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, measured at 450 nm. The dynamic range is > 5000 Cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control, while Crystal Violet staining can be used to determine cell density for normalization. The kit supports measurement of relative levels of phosphorylated Cyclin H in cultured cells and screening of the effects of treatments, inhibitors, or activators on Cyclin H phosphorylation.

According to database references provided with the kit (Gene Symbol CCNH, Gene ID 902, UniProt ID P51946), cyclin H regulates CDK7 as part of the CDK-activating kinase (CAK) complex and is involved in cell cycle control and RNA transcription by RNA polymerase II.