Cyclin B1 Colorimetric Cell-Based ELISA Kit

A cell-based ELISA from Assay Genie that enables qualitative determination of Cyclin B1 protein levels in cultured cells. The assay uses an indirect ELISA format, in which Cyclin B1 is captured by target-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition, with absorbance read at 450 nm.

Designed for use with adherent cells and reactive against human, mouse, and rat samples, the kit operates across a dynamic range of > 5000 Cells and is supplied in a 96-well microplate format with storage at 4°C for 6 months. Normalization of the colorimetric signal is supported by an included anti-GAPDH monoclonal antibody serving as an internal positive control, alongside a Crystal Violet whole-cell staining step that may be used to determine cell density and adjust absorbance values for plating differences.

The lysate-free, high-throughput protocol allows researchers to measure relative Cyclin B1 levels in cells as well as screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Cyclin B1 expression. Cyclin B1 is a regulatory protein central to cell cycle progression—particularly the G2/M transition—and its dysregulation has been implicated in diseases including cancer, making it a relevant biomarker for cell cycle research.