CtBP1 Colorimetric Cell-Based ELISA Kit

A cell-based ELISA from Assay Genie designed for the quantitative measurement of CTBP1 (C-terminal-binding protein 1) levels in cell lysates. The assay uses adherent cells as the sample type and employs a colorimetric detection method measured at 450 nm, providing high sensitivity and specificity for a range of research applications.

The kit is a lysate-free, high-throughput and sensitive assay that can detect the CtBP1 protein expression profile in cultured cells. It can be used to measure the relative amounts of CtBP1 in cells and to screen for the effects of various treatments, inhibitors such as siRNA or chemicals, or activators on CtBP1. Qualitative determination is achieved via an indirect ELISA format: CtBP1 is captured by CtBP1-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP to catalyze a colorimetric reaction upon substrate addition.

Reactivity is confirmed for human, mouse and rat samples, with a dynamic range of > 5000 cells. Normalization is supported by two approaches: a monoclonal antibody specific for human GAPDH is included as an internal positive control, and the Crystal Violet whole-cell staining method can be used to determine cell density. CTBP1 is a key transcriptional co-repressor that modulates gene expression and processes such as cell proliferation, differentiation and apoptosis; its dysregulation has been implicated in diseases including cancer, diabetes and neurological disorders. The kit is supplied in a 96-well microplate format and is stable for 6 months when stored at 4°C.