CSFR Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this cell-based ELISA kit is designed for the colorimetric detection of CSFR (also known as CSF1R) at 450 nm in human samples. The assay uses an indirect ELISA format in which CSFR is captured by CSFR-specific primary antibodies and detected by HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition.

Provided in a lysate-free, high-throughput format on a 96-well microplate, the kit is intended for adherent cells and can be used to compare relative CSFR expression across different treatments, inhibitors, or activators. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining step is provided to assess cell density and adjust for plating differences. The stated dynamic range is greater than 5000 cells per well, and the kit is stored at 4 °C with a stability of 6 months.

The target corresponds to CSF1R (Gene ID: 1436), encoding the macrophage colony-stimulating factor 1 receptor, an oncogenic tyrosine kinase receptor that drives growth and development of monocytes. The assay is qualitative in nature and relies on dual normalization against GAPDH and Crystal Violet cell-density readings rather than yielding an absolute concentration.