CRMP2 (Phospho-Thr514)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the qualitative measurement of CRMP2 phosphorylation at the Thr514 site in cultured cells. The kit uses an indirect ELISA format in which target-specific primary antibodies capture phosphorylated CRMP2, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition.

Reactivity is reported for Human, Mouse and Rat samples, and the assay is described as lysate-free, high throughput and suitable for adherent cells. It can be used to measure relative amounts of phosphorylated CRMP2 in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on CRMP2 phosphorylation. Detection is colorimetric, read at 450 nm, with a stated dynamic range of greater than 5000 cells.

The sub-type is Phospho, and the target gene is DPYSL2 (Gene ID 1808; UniProt ID Q16555). Because the assay is qualitative, two normalization methods are included: a monoclonal anti-GAPDH antibody as an internal positive control for target absorbance values, and Crystal Violet whole-cell staining to determine cell density and adjust for plating differences. The kit is intended for research in developmental biology.