CREB3L2 Transcription Factor Activity Assay

The Assay Genie CREB3L2 Transcription Factor Activity Assay quantifies DNA-binding activity of the BBF2H7 transcription factor in nuclear and cell lysates, supporting research into endoplasmic reticulum stress signaling and the unfolded protein response element (UPRE) pathway. Validated for human, mouse, and rat samples, this microplate-based kit enables investigation of CREB3L2 activation under varying cellular conditions, including studies of collagen biosynthesis regulation and low-grade fibromyxoid sarcoma biology where CREB3L2 gene fusions have been characterized.

Reagents should be stored at -20°C and protected from light; aliquot binding components to minimize freeze-thaw cycles and preserve binding specificity across repeated runs. Researchers studying ER-associated transcriptional regulation, secretory pathway load, and transcription factor cleavage events mediated by Site-1 and Site-2 proteases will find this assay useful for measuring nuclear translocation and DNA engagement of endogenous CREB3L2.