CREB (Phospho-Ser133)Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed for the accurate quantification of CREB phosphorylation at Ser133 in cell lysates. It uses a colorimetric detection method (450 nm) with high sensitivity and specificity, allowing precise measurement of CREB activation in response to various stimuli. The assay is reactive with Human, Mouse, and Rat samples, has a dynamic range of > 5000 Cells, and is suitable for adherent cells.

The kit employs a convenient, lysate-free, high-throughput indirect ELISA format. CREB (Phospho-Ser133) is captured by target-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalised to cell amounts.

Researchers can use this kit to measure the relative amounts of phosphorylated CREB in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on CREB phosphorylation, making it a valuable tool for studying the signalling pathways involved in CREB activation and its impact on cellular function.