CREB (Phospho-Ser121)Transcription Factor Activity Assay

Assay Genie offers a colorimetric, DNA-binding ELISA kit for the CREB (Phospho-Ser121) Transcription Factor Activity Assay. The phospho-Ser121 format is intended for nuclear and cell lysates, with reactivity to human, mouse and rat samples.

The manufacturer's CREB transcription factor activity assay format supports quantitative measurement of CREB activity in cell lysates or tissue samples, as well as detection and qualitative analysis of endogenous activated transcription factor levels in nuclear and cell lysates. Its stated assay time is "4.5 hours", with colorimetric detection at "450 nm". CREB (cAMP response element-binding protein) binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters, and stimulates transcription on binding to the CRE. Transcription activation is enhanced by TORC coactivators, which act independently of Ser-133 phosphorylation. CREB regulates gene expression in response to growth factors, stress and neurotransmitters, and its activity is involved in cell proliferation, differentiation and survival. Dysregulation of CREB activity has been implicated in cancer, neurological disorders and metabolic disorders.