Cox1 Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the quantification of Cyclooxygenase 1 (COX1) levels in cell lysates and tissue homogenates, using a 96-Well Microplate format with detection at 450 nm. The kit is reactive with human samples and validated for adherent cells, offering a lysate-free, high-throughput workflow for measuring relative Cox1 expression in cultured cells and for screening the effects of various treatments, inhibitors, or activators.

The assay employs an indirect ELISA format in which Cox1-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values. Following the colorimetric measurement, a Crystal Violet whole-cell staining method may be used to determine cell density, allowing results to be normalized to cell amounts to adjust for plating differences.

COX1 (gene symbol PTGS1, UniProt ID: P23219, molecular weight 72,010 Da) is a key enzyme involved in prostaglandin production, playing critical roles in inflammation, pain, and other physiological processes. Dysregulation of COX1 has been implicated in various diseases, including cancer, inflammatory disorders, and cardiovascular conditions, highlighting its importance as a potential therapeutic target. The kit has a dynamic range of > 5000 Cells, is stored at 4°C for 6 Months, and is categorized under the Metabolism research area.