CORT (Corticosterone) ELISA Kit (UNFI0096)

This competitive immunoassay from Assay Genie enables quantitative measurement of corticosterone, a primary adrenal glucocorticoid and a widely used biomarker of hypothalamic-pituitary-adrenal (HPA) axis activation. The ELISA procedure relies on colorimetric readout following enzymatic substrate conversion, with a pre-coated microplate and corticosterone-specific antibody supplied for direct sample analysis. The kit's universal reactivity supports cross-species compatibility, and the assay accommodates small sample volumes with a serial-dilution standard set for constructing the calibration curve used to interpolate unknown concentrations.

Corticosterone quantification supports research applications including acute and chronic stress paradigms, circadian rhythm characterization, adrenalectomy verification, glucocorticoid receptor signaling studies, and metabolic or neuroendocrine disorder models. Store kit components refrigerated and protect from light to preserve antibody-binding activity and enzyme conjugate stability; bring all reagents to room temperature prior to initiating the assay run.