Connexin 43 (Phospho-Ser367)Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this colorimetric cell-based ELISA kit enables accurate detection and quantification of Connexin-43 phosphorylation at Serine 367 in cell-based assays. The lysate-free, high-throughput assay measures the relative amounts of phosphorylated Connexin-43 in cultured cells and can be used to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Connexin-43 phosphorylation.

Qualitative determination is achieved through an indirect ELISA format in which Connexin 43 (Phospho-Ser367) is captured by phospho-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody and catalyze a colorimetric reaction upon substrate addition, measured at 450 nm. Designed for adherent cell samples and reactive with human, mouse, and rat species, the kit has a dynamic range of greater than 5000 cells and includes a monoclonal GAPDH antibody as an internal positive control for normalizing target absorbance values, plus a Crystal Violet whole-cell staining method for determining cell density. It is a reliable resource for studying gap junction regulation and its implications in various physiological and pathological conditions.