Collagenase Type V, 5 g

Lyophilized enzyme preparation supplied as a 5 g bottle for research workflows requiring gentle dissociation of connective tissue. STEMCELL Technologies Collagenase Type V combines primary collagenase activity with supplemental caseinase, clostripain, and tryptic activities, making it effective for digesting the extracellular matrix of dense, fibrous organs including pancreas, lung, liver, and adipose tissue. Researchers commonly reconstitute the powder in balanced salt solution or culture medium and titrate working concentrations between 0.5 and 2 mg/mL depending on tissue hardness, cell yield targets, and incubation time at 37 degrees C. The formulation supports downstream applications such as primary cell isolation, organoid seeding, and stromal vascular fraction preparation, and pairs with DNase I and protease inhibitors when minimizing damage to surface markers is critical. Each lot is characterized by enzymatic activity assays and is intended for in vitro research use only. Store the dry powder refrigerated and protected from moisture, and aliquot reconstituted stocks for single use to limit freeze-thaw cycles.