Collagenase Type IV, 100 mg

Lyophilized Collagenase Type IV from STEMCELL Technologies is a 100 mg enzyme preparation derived from Clostridium histolyticum, formulated for efficient enzymatic dissociation of connective tissue in primary cell isolation workflows. The 100 mg vial format supports flexible reconstitution, allowing researchers to prepare working stocks at concentrations typically ranging from 0.5 to 2.5 mg/mL depending on tissue density and target cell type. Each batch is verified for protease activity of ≥160 U/mg, ensuring reproducible tissue digestion across experiments. The lyophilized format remains stable for extended periods when stored at -20 degrees C, with minimal loss of enzymatic activity following reconstitution. Researchers commonly combine Collagenase Type IV with supplementary enzymes such as elastase, DNase I, or trypsin to tailor dissociation protocols for challenging tissues. Provided as a single-use 100 mg vial, the product integrates into established protocols for organoid preparation, single-cell suspension generation, hepatocyte isolation, adipocyte harvesting, lung parenchyma dissociation, and downstream flow cytometry or cell culture applications. Manufactured under ISO quality standards for research use only.