Collagenase Type IV, 1 g

Enzymatic dissociation of animal tissues in primary cell isolation workflows is the primary application for this lyophilized Collagenase Type IV enzyme preparation, supplied by STEMCELL Technologies as a 1 g powder. The blend retains clostripain and tryptic activities alongside its primary collagenase activity, supporting effective breakdown of the extracellular matrix in liver, lung, adipose, and cardiac tissues. A typical working concentration ranges from 0.5 to 2.5 mg/mL, prepared in a calcium-containing balanced salt solution immediately before use.

Researchers commonly pair Collagenase Type IV with DNase I during single-cell preparations to minimize cell clumping and improve yield when isolating hepatocytes, stromal vascular fractions, and other primary cell populations. Reconstituted stocks should be aliquoted and stored at -20 °C; frozen aliquots retain enzymatic activity across several freeze-thaw cycles for downstream cell culture and flow cytometry workflows. Each bottle supplies enough material for roughly 20 to 100 small-scale digestions depending on tissue mass and enzyme loading. The product is intended for research use only.