Collagenase Type III, 1 g

This lyophilized enzymatic preparation from STEMCELL Technologies is specifically formulated for tissue dissociation and cell isolation workflows. Collagenase Type III is derived from Clostridium histolyticum and exhibits high collagenase activity with minimal tryptic activity, making it particularly effective for breaking down dense connective tissue matrices. Researchers commonly apply this enzyme at working concentrations of 1-2 mg/mL in calcium-containing buffers to digest collagen-rich tissues such as adipose, liver, lung, and tumor specimens. Each vial contains 1 g of the freeze-dried powder, which should be reconstituted in sterile buffer immediately before use and stored desiccated at -20°C for long-term stability. The enzyme is widely used to generate single-cell suspensions for downstream applications including primary cell culture, flow cytometry analysis, and transplantation studies.