Collagenase Type II, 5 g

Tissue dissociation labs working with collagen-rich organs rely on STEMCELL Technologies Collagenase Type II, supplied as a 5 g vial of lyophilized enzyme preparation derived from Clostridium histolyticum. The active enzymes cleave native collagen fibrils and are widely used in cell isolation and cell culture protocols, including perfusion of liver for hepatocyte harvest, enzymatic digestion of pancreatic tissue for islet isolation, and dissociation of adrenal, lung, and other connective-tissue-dense organs. Each lot is characterized by activity assays so researchers can benchmark digestion kinetics against published type II protocols. The dry format supports long shelf life when refrigerated and protected from light, and reconstituted aliquots remain active for routine use after short-term storage. Recommended working concentrations fall between 0.5 and 2 mg/mL depending on tissue density and target cell yield, allowing flexible protocol tuning for adherent or suspension cell culture downstream. Researchers pair it with DNase I and a compatible buffer such as Hanks' Balanced Salt Solution to reduce clumping and improve single-cell viability. This 5 g pack suits high-throughput organ harvest labs running multiple dissociations per week, including repeated digestions of mouse or rat tissue for primary cell culture studies.