Collagenase/Hyaluronidase, 10mL

Primary cell isolation from intact tissue relies on gentle enzymatic disruption of the extracellular matrix. This 10 mL Collagenase/Hyaluronidase vial from STEMCELL Technologies delivers a combined collagenase and hyaluronidase activity that digests collagen fibrils and hyaluronic acid-rich basement membrane components, releasing stromal cells, fibroblasts, and other resident populations from biopsy specimens including tumor, skin, lung, and intestinal mucosa samples.

Researchers typically dilute the working stock between 1x and 10x into serum-free buffer, depending on tissue density and target cell yield, and commonly supplement with DNase I to reduce viscosity caused by released genomic DNA. The 10 mL format supports multiple small-scale digestions of biopsy-grade samples. Storage at -20°C is recommended, with single-use aliquots prepared to preserve enzymatic activity across freeze-thaw cycles.

Downstream applications include flow cytometry analysis of dissociated populations, in vitro culture expansion, and preparation of single-cell suspensions for downstream assays. Each lot is manufactured under defined activity specifications to support reproducible dissociation performance across independent experiments.