Collagen XX alpha1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit enables measurement of Collagen XX alpha1 protein expression directly in cultured cells using a lysate-free, indirect ELISA workflow. The assay uses target-specific primary antibodies with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition, with absorbance read at 450 nm. A monoclonal Anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance, and a Crystal Violet whole-cell staining step is used to account for cell density differences between wells.

Reactivity is reported for Human, Mouse, and Rat samples, and the stated dynamic range covers measurements above 5,000 cells per well in the 96-well microplate format. The kit is suited to assessing relative Collagen XX alpha1 levels in adherent cell cultures and to screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators. Storage stability is given as 6 months at 4°C. According to the manufacturer, dysregulation of collagen XX alpha1 has been linked to diseases such as osteoarthritis and cardiovascular disorders, making it a useful biomarker for related research.

Associated target information: gene symbol COL20A1 (Gene ID 57642; UniProt Q9P218), described as a probable collagen protein, with chromosomal location 20q13.33 and a reported molecular weight of 138,781 Da.