Collagen XI alpha1 Colorimetric Cell-Based ELISA Kit

Assay Genie provides a cell-based ELISA kit for the accurate measurement of collagen XI alpha1 levels in cell culture supernatants. The product targets collagen XI alpha1 (COL11A1), a key component of extracellular matrix synthesis and organisation that plays a vital role in tissue development, maintenance, and repair. Dysregulation of collagen XI alpha1 expression has been implicated in various pathological conditions, including arthritis, skeletal disorders, and connective tissue diseases, making it a valuable biomarker for studying these diseases and developing potential treatments.

The kit employs a colorimetric detection method at 450 nm with a stated dynamic range of > 5000 cells and shows reactivity with human and mouse samples. It is a convenient, lysate-free, high-throughput and sensitive assay for detecting the collagen XI alpha1 protein expression profile in cultured cells, suitable for measuring the relative amounts of collagen XI alpha1 and for screening the effects that various treatments, inhibitors, or activators have on the target. Qualitative determination is achieved through an indirect ELISA format: collagen XI alpha1 is captured by specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyse a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density. The kit is designed for use with adherent cells, with specified storage at 4°C for 6 months.