Collagen V alpha1 Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie enables qualitative measurement of Collagen V alpha1 (COL5A1) protein expression in cultured adherent cells, with applications in extracellular matrix research where dysregulation of this fibrillar collagen has been implicated in fibrosis, cancer, and autoimmune disorders. The kit uses a convenient, lysate-free, high-throughput indirect ELISA format compatible with human samples, allowing researchers to screen the relative effects of treatments, inhibitors (such as siRNA or chemicals), or activators on Collagen V alpha1 levels.

Detection is colorimetric, read at 450 nm, on a microplate format with a stated dynamic range of greater than 5000 cells. The kit is stored at 4 °C with a stability of 6 months. Because the assay is qualitative, two normalisation methods are supplied: a monoclonal anti-human GAPDH antibody acts as an internal positive control, and a Crystal Violet whole-cell staining step is used to determine cell density, allowing absorbance values to be normalised to cell amounts.

In the workflow, Collagen V alpha1 is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primaries, catalysing a colour reaction upon substrate addition. Reference identifiers for the target include Gene ID 1289, UniProt ID P20908, OMIM 120215, and Unigene Hs.210283.